The Technical Vault
By SOSCleanroom
250 mL HDPE
Wide Mouth
43-410 PP Closure
Linerless Double Seal
72 per Case
Not Autoclavable
WHEATON 209548: qualify the bottle, closure, contents, and conditions together
The WHEATON® 209548 is a
250 mL natural HDPE wide-mouth bottle supplied with a
polypropylene screw closure. Its linerless double-seal
design, non-drip pour lip, and defined packaging make
it a practical container for compatible laboratory liquids.
Successful selection depends on more than capacity.
The closure, liquid chemistry, temperature, exposure time,
cleanliness requirements, and handling conditions must
all be appropriate for the intended application.
Operations takeaway:
A water-based leak-test statement does not establish
universal chemical compatibility, a working-pressure
rating, sterility, or approval of an entire transport
package. Use the manufacturer's data as an input to
the facility's application qualification.
Core product data for purchasing and receiving
| Attribute |
209548 specification |
| Manufacturer / brand |
DWK Life Sciences / WHEATON® |
| Catalog number |
209548 |
| Capacity |
250 mL nominal; manufacturer ounce-size designation 8 oz |
| Bottle |
Natural HDPE; wide mouth |
| Closure |
Included polypropylene screw cap; 43-410 thread finish; linerless double seal |
| Bottle dimensions |
61 mm diameter; 125 mm height |
| Packaging |
72 bottles per case; inner-pack quantity 12 |
| Autoclavable |
No |
Capacity interpretation:
The 8 oz entry is the manufacturer's size designation,
not an exact conversion of 250 mL. No brim capacity,
recommended working-fill volume, or volumetric accuracy
is established in the reviewed item data.
Closure selection: 43-410 identifies the finish, not the whole seal
The manufacturer describes a linerless double-seal
closure. This differs from a bottle that depends
on a separately specified foam or film liner. Preserve
the matched bottle-and-cap combination when evaluating
sealing performance.
The 43-410 thread designation should not be treated as
proof that any cap with the same designation reproduces
the original seal. Confirm replacement-cap suitability
with DWK rather than relying on thread fit alone.
Unspecified parameters:
The reviewed item records do not provide an application
torque, removal torque, clear mouth diameter, or
approved number of reuse cycles. Do not assign
numerical values without manufacturer support.
The molded bead provides a location for a shrink band.
A bead alone is not a tamper-evident seal, and the
reviewed 209548 configuration does not identify a
shrink band as included.
Leak resistance and the manufacturer's water-based test
DWK's bottle-family literature references pressure testing
including 95 kPa and states that the
leakage results are based on water.
It also instructs customers to evaluate leak resistance
with their specific liquids and storage or transport
conditions.
The practical qualification question is whether the
supplied bottle and cap maintain the required containment
with the actual contents, fill level, closure procedure,
orientation, time, and temperature cycle.
Pressure-test boundary:
Do not interpret a leakage test as a continuous
working-pressure rating, vacuum rating, centrifuge
rating, or permission to use the bottle as a pressure
vessel. Those applications are not established by
the reviewed 209548 documentation.
Chemical compatibility: review HDPE and polypropylene separately
The bottle and cap use different polymers. Screen the
proposed contents against HDPE and
polypropylene, including concentration,
contact temperature, exposure duration, and any mixed
formulation.
DWK's compatibility calculator provides separate polymer
ratings at 20°C and 50°C. It is a screening
resource, not a complete filled-package qualification
or proof of sample stability.
Review DWK's Chemical Compatibility Calculator
Sample-quality checkpoint:
Determine whether the method also requires extractables,
adsorption, permeation, light-protection, or cleanliness
data. No analytical-recovery or universal chemical-
compatibility claim is added for this bottle.
Freezing, closure temperature limits, and autoclaving
The product page and manufacturer item files state HDPE
suitability for freezing at -100°C.
Separately, DWK's general resin guide lists
-20°C as the lower end of its
generic polypropylene temperature range.
These statements describe different levels of information
and do not establish a complete low-temperature protocol
for the filled bottle and supplied PP cap. Obtain
application-specific confirmation for the intended
freezer temperature, headspace, handling, and freeze-thaw
cycle.
Explicit product restriction:
WHEATON 209548 is listed as not autoclavable.
General information about autoclavable polypropylene
must not be applied to the complete HDPE bottle.
No liquid-nitrogen immersion qualification is
established in the reviewed item records.
Read DWK's general plastic resin guidance
Laboratory-pack configuration versus bulk packaging
Catalog 209548 is the 72-count configuration, with an
inner-pack quantity of 12 and an included polypropylene
cap. The family description also discusses bulk formats
with bottles and closures bagged separately.
The bulk-format references to double bagging, twist ties,
and reduced particulate should not automatically be
applied to this laboratory-pack item. Confirm the exact
supplied packaging if bag layers, closure presentation,
or material-transfer controls are purchasing requirements.
Sterility, cleanroom use, and documentation limits
The reviewed manufacturer records do not establish
a supplied sterile designation, sterility assurance
level, endotoxin limit, cleanroom class, or quantified
particle-release specification for 209548.
Blank fields in a product-information file are not
certifications. Neither leak resistance nor reduced-
particulate packaging language establishes sterile
or aseptic suitability.
Qualification checkpoint:
Define the required cleanliness, preparation,
packaging, material transfer, and supporting
documentation before introducing this bottle into
a controlled environment. Request product-specific
certificates where necessary rather than relying
on general brand or facility credentials.
Transport use: a primary receptacle is not a complete shipping system
DWK identifies primary-receptacle transport applications
for this bottle family. That does not certify an
assembled shipment containing a particular chemical
or specimen.
For example, U.S. Category B infectious-substance rules
require a primary receptacle, secondary packaging,
and rigid outer packaging. Liquid shipments have
additional containment and absorbent requirements;
air shipments include a 95 kPa pressure-differential
requirement for the primary receptacle or secondary
packaging.
The responsible shipper must determine the classification
and complete packaging requirements for the contents
and transport mode.
Review 49 CFR 173.199
.
Inspection, filling, storage, and reuse controls
- Inspect the assembly: Check for deformation, cracks, damaged threads, sealing-surface defects, and contamination.
- Control the closure: Keep the matched cap and sealing surfaces clean; use an approved closure procedure.
- Define the fill: Establish the working volume and headspace for the actual contents and temperature conditions.
- Verify containment: Qualify the filled assembly for the intended orientation, storage duration, and handling.
- Maintain identification: Label contents and apply the laboratory's tracking and segregation requirements.
- Control reuse: Approve cleaning and reuse only when supported by the application and manufacturer guidance; do not assume an unlimited reuse life.
The manufacturer's 2026 setup file lists room-temperature
shipping and storage conditions for the supplied product
and records shelf life as N/A. That does not establish
the storage life of a filled sample or an indefinite
service-life guarantee.
Technical FAQs
Is the cap included?
Yes. The 209548 item is identified with a 43-410
polypropylene cap. Do not substitute a different
closure without confirming the required seal.
Is the bottle autoclavable?
No. Both the manufacturer's item data and product
listing identify this HDPE bottle as not autoclavable.
Is it guaranteed leakproof with every liquid?
No universal claim is supported. DWK identifies
water-based testing and requires qualification
for the actual liquid and conditions.
Does 43-410 mean the opening is 43 mm wide?
It is the thread-finish designation. A clear
internal mouth diameter is not established in
the reviewed product data.
Is the bottle supplied sterile?
The reviewed records do not establish a supplied
sterile designation. Do not represent it as
sterile without supporting product-specific documentation.
How many bottles are supplied?
72 bottles per case, with an inner-pack quantity
of 12. Confirm the order unit before comparing
case and individual-bottle pricing.
Manufacturer documentation and technical references
Source basis:
Product specifications were cross-checked against
manufacturer-supplied product-information and 2026
item-setup records. General polymer guidance and
regulatory references are identified separately.
Application checkpoints are facility-selection guidance,
not additional product certifications.
If you have any questions please email us at Sales@SOSsupply.com
or give us a call at (214) 340-8574.
For additional product guidance, use the SOSCleanroom AI ChatBot
or contact our team for manufacturer documentation assistance.
Technical insight:
Qualify the complete bottle-and-closure assembly with
the intended contents and conditions. Capacity, resin
type, and a water-based leak test are only part of
the selection.
Last updated: October 22, 2026
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