DWK 227498 WHEATON BOD Bottle, 300mL Without Stopper, Un-numbered

The WHEATON® 227498 is a 300 mL USP Type I borosilicate glass bottle designed for biochemical oxygen demand testing in water and wastewater laboratories. Its distinctive shoulder and flared-mouth geometry are intended to reduce air entrapment and help maintain a water seal during incubation when a suitable glass stopper is installed.

The bottle is supplied without a stopper, without a manufacturer-assigned number sequence, and without a barcode. A permanently screened-on writing patch is provided for laboratory identification.

The published dimensions describe the stopper-free bottle configuration. The 300 mL designation is nominal; it does not establish a calibrated Class A volumetric tolerance.

Biochemical oxygen demand describes the amount of dissolved oxygen consumed by microorganisms as they biologically break down oxygen-demanding material in a water sample.

In conventional five-day BOD testing, an initial dissolved oxygen measurement is compared with a measurement after the sample or a prepared dilution has been incubated under prescribed conditions.

Five-day BOD testing commonly uses five days of incubation at 20°C in darkness. The laboratory must apply its approved method for dilution water, blanks, seeding, sample preparation, dissolved oxygen measurement, and result calculation.

For BOD testing, unwanted oxygen entering or remaining in the sample container can interfere with the intended measurement. WHEATON addresses this issue through the geometry of its BOD bottle family.

Proper filling technique, stopper fit, and inspection for trapped bubbles remain necessary even with the specialized bottle design.

WHEATON BOD bottles feature a flared mouth designed to hold a water seal around the glass stopper during incubation.

The water-seal arrangement helps prevent ambient air from entering the bottle and influencing the dissolved oxygen conditions during the test.

A glass stopper must be correctly fitted to the bottle before this arrangement can function as intended. The stopper is not part of the 227498 purchasing configuration.

DWK identifies replacement USP Type I borosilicate glass stoppers that fit the WHEATON 60 mL and 300 mL BOD bottle families.

Both styles belong to WHEATON's BOD replacement-stopper range. The selected style should match the intended handling and measurement procedure.

These are separately cataloged WHEATON configurations. The manufacturer publishes different heights for the stopper-free and stopper-included products. Do not use the dimensions or included-component descriptions from another SKU for 227498.

Catalog 227498 is listed as unnumbered and not barcoded. It does, however, include the manufacturer's large, permanently screened-on writing patch.

The writing patch can support laboratory-applied sample identifiers, date markings, or other information required by the laboratory's sample-tracking process.

The unnumbered configuration should not be represented as carrying a factory-applied serialized number, barcode, or unique sample identifier.

WHEATON recommends its 300 mL BOD bottle format for the five-day BOD method referenced in Standard Methods, 21st Edition, Method 5210 B (2005).

A conventional BOD5 workflow generally includes the following operations, as specified by the applicable testing method:

1. Prepare the sample, dilution water, blanks, and any required seed or quality-control materials.
2. Fill the appropriate BOD bottles carefully to minimize trapped air.
3. Measure initial dissolved oxygen according to the approved procedure.
4. Install a compatible stopper and establish the required water seal without entrapping air.
5. Incubate under the method's specified conditions, commonly five days at 20°C in darkness for BOD5.
6. Measure final dissolved oxygen and evaluate the applicable blanks, dilutions, and quality controls.
7. Calculate and report the result according to the approved method.

These are practical handling and quality-control considerations. They are not quantified product defect rates or performance claims.

WHEATON identifies its BOD bottle family as manufactured from USP Type I borosilicate glass. Borosilicate glass is commonly selected for laboratory applications where chemical durability and resistance to aqueous attack are important.

The manufacturer's technical glass reference describes borosilicate glass types and their chemical and physical properties. However, general properties of a glass type do not automatically establish all performance limits for a finished BOD bottle.

Accurate BOD testing requires appropriately prepared glassware. Contamination or residual materials can contribute to analytical interference, so cleaning procedures should be selected according to the laboratory's approved BOD method.

DWK's general glassware guidance recommends careful handling, suitable non-abrasive cleaning, thorough rinsing, and inspection before reuse.

• Inspect before use: Reject bottles with chipped mouths, cracks, or other visible damage.
• Prevent scratching: Avoid abrasive cleaning tools that may weaken the glass surface.
• Remove residues: Follow the BOD method's cleaning requirements and avoid introducing oxygen-demanding contamination.
• Rinse appropriately: Use the rinse-water quality specified by the laboratory procedure.
• Inspect stopper interfaces: Keep the flared mouth and glass-joint surfaces free from damage.
• Maintain traceability: Identify prepared bottles according to laboratory quality procedures.

No catalog-specific validated autoclaving, depyrogenation, or sterilization cycle is established for 227498 in the reviewed product records.

WHEATON 227498 is supplied in cases of 24 stopper-free BOD bottles. Receiving personnel should verify the correct configuration before releasing the product for laboratory use.

• Identity: Confirm manufacturer SKU 227498 and 300 mL nominal capacity.
• Quantity: Confirm 24 bottles per case.
• Stopper status: Confirm that no stopper is expected with this item.
• Glass condition: Inspect the bottles for breakage, cracks, and chipped mouths.
• Configuration: Confirm unnumbered, non-barcoded bottles with permanent writing patches.
• Separate components: Verify that any required robotic or pennyhead stoppers have been ordered independently.
• Method readiness: Confirm that the selected bottle-and-stopper combination meets the laboratory's BOD testing requirements.

The following technical references provide additional information about laboratory glass, glassware handling, cleaning, and biochemical oxygen demand. None are DWK product purchasing pages.

Method reference: DWK identifies Standard Methods for the Examination of Water and Wastewater, 21st Edition, Method 5210 B (2005). Laboratories should consult the applicable method edition and their approved procedures for current testing requirements.

Documentation boundary: General technical references do not establish lot-specific product certification, a calibrated bottle-volume tolerance, or a validated BOD method for every laboratory system.

SOSCleanroom can assist with WHEATON BOD bottle ordering, robotic and pennyhead replacement stopper selection, unnumbered and numbered configurations, and available manufacturer technical documentation.