The Technical Vault
By SOSCleanroom
WHEATON 227497-00
300 mL BOD Bottle
USP Type I Glass
Robotic Glass Stopper
Water-Seal Mouth
Unnumbered
No Barcode
24 / Case
WHEATON 227497-00: bottle geometry engineered for biochemical oxygen demand testing
The WHEATON® 227497-00 is a 300 mL biochemical oxygen demand (BOD) incubation bottle manufactured by DWK Life Sciences from USP Type I borosilicate glass. It includes an interchangeable robotic-style ground-glass stopper and is supplied in an unnumbered, non-barcoded configuration.
The bottle's geometry is designed around three practical BOD testing challenges: minimizing trapped air during filling, reducing air entrapment beneath the stopper, and maintaining a water seal to limit atmospheric oxygen entry during incubation.
DWK identifies this 300 mL format for conventional five-day BOD testing, referencing Standard Methods for the Examination of Water and Wastewater, Method 5210B. The manufacturer lists a 69 mm diameter, 165 mm height, and a case quantity of 24.
Operations takeaway: BOD bottles function as part of a controlled analytical method. The sample, dissolved oxygen measurement, air-bubble control, incubation conditions, water seal, and laboratory quality controls all affect the final result. Proper bottle design supports the method but cannot compensate for incorrect handling or testing procedures.
Specifications (SKU controls for purchasing, SOPs, and receiving)
- Manufacturer: DWK Life Sciences
- Brand: WHEATON®
- Catalog Number: 227497-00
- Product Family: WHEATON BOD Bottles
- Nominal Capacity: 300 mL
- Bottle Material: Borosilicate glass
- Manufacturer Glass Classification: USP Type I
- Diameter: 69 mm
- Manufacturer-Listed Height: 165 mm
- Stopper Included: Yes
- Stopper Style: Glass Robotic
- Stopper Geometry: Tapered bottom, interchangeable glass stopper
- Replacement Stopper: WHEATON 227670
- Neck Design: Flared mouth for water-seal formation
- Shoulder: Special radius designed to help expel air during filling
- Identification: Permanently screened-on writing patch
- Number Sequence: Unnumbered
- Barcoding: No
- Case Quantity: 24 bottles
- Primary Method Context: Five-day BOD, Standard Methods 5210B
- Related Measurement: Dissolved oxygen measurement using an appropriate method and compatible instrument
Specification control: 227497-00 is the unnumbered robotic-stopper configuration. It is not the 227497-00G pennyhead-stopper bottle, the 227498 bottle without stopper, or the 227497-01 pre-numbered/barcoded configuration.
Biochemical oxygen demand: what the five-day test measures
Biochemical oxygen demand is a measure of the dissolved oxygen consumed by microorganisms as they metabolize biodegradable organic material under controlled test conditions. Results are generally reported as oxygen demand in milligrams of oxygen per liter of sample (mg/L).
The conventional BOD5 procedure involves measuring dissolved oxygen at the beginning of a test, incubating the sample or prepared dilution for five days in the dark at a controlled temperature, and determining the remaining dissolved oxygen at the end of incubation.
- Initial Dissolved Oxygen: Oxygen concentration measured at the start of the method-defined test period.
- Final Dissolved Oxygen: Oxygen concentration measured after incubation.
- Oxygen Depletion: Reduction in dissolved oxygen during the test.
- Dilution: The sample may be diluted to maintain appropriate oxygen conditions.
- Microbial Activity: Biological consumption of dissolved oxygen contributes to the result.
- Calculation: The actual reported value depends on the method, including applicable dilution, seed, and blank corrections.
BOD is an empirical biological test rather than a direct total-organic-carbon measurement. It should not be treated as interchangeable with chemical oxygen demand (COD), total organic carbon (TOC), or an individual dissolved oxygen concentration.
Method takeaway: A 300 mL BOD bottle provides the physical incubation vessel. The applicable method controls sample dilution, seeding, dissolved oxygen measurement, incubation, quality-control checks, and calculation. Use the method revision required by the laboratory or regulatory program.
Five-day BOD testing: incubation conditions and workflow checkpoints
Conventional five-day BOD testing is performed using a defined incubation period and controlled temperature. A commonly referenced condition is five days at 20°C in the dark, with actual tolerances and acceptance criteria determined by the applicable procedure.
- Prepare laboratory glassware: Clean, rinse, and inspect BOD bottles and stoppers according to the approved method.
- Prepare the sample: Apply the prescribed sampling, holding, dilution, seeding, nutrient, and pretreatment steps.
- Fill the bottle: Avoid trapped air and excessive agitation that could change dissolved oxygen.
- Seat the stopper: Confirm correct fit without leaving visible air bubbles in the sample.
- Establish the water seal: Follow the approved method for filling and maintaining the flared-mouth water-seal area.
- Measure initial DO: Use an approved dissolved oxygen measurement procedure.
- Incubate: Maintain the applicable five-day temperature and dark conditions.
- Measure final DO: Determine oxygen remaining at the end of incubation.
- Evaluate quality controls: Apply the procedure's dilution water blanks, seed controls, checks, and sample acceptance criteria.
- Calculate and record: Report the method-defined BOD result with relevant corrections and quality-control documentation.
This overview describes the analytical workflow rather than a complete standard operating procedure. The current governing test method determines the exact sequence, timing, dissolved oxygen measurement approach, and validity criteria.
Air-entrapment control: why the bottle shoulder and tapered stopper matter
BOD measurement can be affected by oxygen introduced from trapped air. Even a small bubble introduces an additional gas phase that can alter the sample's oxygen conditions during incubation.
DWK designs the WHEATON BOD bottle with a specially shaped shoulder radius intended to sweep air out during filling. The included glass stopper has a tapered lower profile designed to reduce the potential for air entrapment as it is seated.
- Shoulder Radius: Helps displace air toward the bottle opening during filling.
- Tapered Stopper Bottom: Intended to reduce trapped air beneath the seated stopper.
- Ground-Glass Joint: Provides the mating interface between the bottle and stopper.
- Correct Filling: Remains necessary to avoid visible bubbles and sample disturbance.
- Stopper Inspection: Chips, contamination, or damaged ground surfaces can interfere with proper seating.
Air-bubble checkpoint: The bottle geometry is intended to minimize entrapment, not to guarantee a bubble-free sample automatically. Operators must still verify proper filling and stopper seating.
Flared-mouth water seal: limiting atmospheric oxygen entry during incubation
WHEATON BOD bottles have a flared mouth that accommodates a ring of water around the installed glass stopper. When established and maintained according to the test method, this water seal helps reduce the risk of outside air being drawn into the bottle during incubation.
The water seal is important because oxygen entering a bottle from the surrounding atmosphere can alter dissolved oxygen measurements and interfere with interpretation of oxygen consumed by biological activity.
- Flared Mouth: Creates the space for water to surround the seated stopper.
- Water Barrier: Helps limit air movement into the bottle under the prescribed incubation conditions.
- Water Level: Must be maintained as required by the approved method.
- Evaporation: A protective cover may be specified by a method to reduce loss of the water seal during incubation.
- Stopper Fit: Proper stopper seating remains essential to the intended bottle performance.
- Incubation Inspection: Check for loss of the water seal or other problems according to the laboratory procedure.
Control point: The flared mouth provides the geometry for a water seal. It is not an automatic airtight mechanism and does not establish a pressure-rated or independently validated leakproof closure. The water seal must be correctly prepared and maintained.
Robotic glass stopper versus pennyhead stopper: choosing the correct configuration
DWK manufactures WHEATON BOD bottles with glass robotic and glass pennyhead stopper configurations. Both use tapered glass stoppers intended to reduce air entrapment, but the upper stopper geometry differs.
Catalog 227497-00 includes the glass robotic stopper. The manufacturer also offers the corresponding unnumbered 300 mL bottle with a glass pennyhead stopper under catalog 227497-00G.
| Attribute |
227497-00 |
227497-00G |
| Capacity |
300 mL |
300 mL |
| Stopper included |
Glass Robotic |
Glass Pennyhead |
| Diameter |
69 mm |
69 mm |
| Height |
165 mm |
165 mm |
| Numbering |
Unnumbered |
Unnumbered |
| Barcode |
No |
No |
| Replacement stopper |
227670 |
227672 |
| Case quantity |
24 |
24 |
The manufacturer's robotic designation describes the stopper style and intended handling configuration. It does not, by itself, guarantee compatibility with every automated BOD analyzer or robotic sample handling system.
Stopper selection: Choose the robotic or pennyhead configuration based on the approved bottle handling and instrument requirements. Do not substitute the pennyhead replacement part when the procurement specification calls for the robotic stopper.
Dissolved oxygen meters and probe compatibility
DWK describes the WHEATON BOD bottle stopper joint as compatible with the probes of leading meters used for BOD and dissolved oxygen measurement. This product positioning is relevant to laboratories using DO probes directly with BOD incubation bottles.
However, probe dimensions, shaft length, insertion geometry, sample displacement, measurement technique, and instrument configuration vary by manufacturer and model. The reviewed 227497-00 listing does not provide a complete list of approved meters or probes.
- Probe Fit: Confirm the instrument probe and the applicable bottle/stopper joint geometry.
- Probe Insertion: Follow the instrument manufacturer's method to avoid damage or unintended sample aeration.
- Measurement Displacement: Account for liquid displacement or required filling conditions under the approved procedure.
- Instrument Calibration: Verify DO meter readiness and calibration according to the analytical method.
- Robotic Handling: Confirm the specific bottle and stopper geometry with automated sample-handling equipment.
- Alternative DO Methods: Use the test method's permitted dissolved oxygen procedure rather than assuming a specific probe is included.
Instrument compatibility boundary: This product does not include a dissolved oxygen meter, probe, automated analyzer, or validated interface to a particular instrument. Verify compatibility before purchasing for a specific system.
300 mL capacity: nominal size, actual liquid volume, and bottle dimensions
The manufacturer specifies a 300 mL capacity for WHEATON 227497-00. This is the published nominal bottle size and should not be interpreted as an individually certified exact working volume.
For methods requiring actual bottle-volume determination, the laboratory should follow the prescribed procedure. The product listing does not provide a specific calibrated volume tolerance or brimful capacity for this SKU.
- Nominal Capacity: 300 mL
- Outside Diameter: 69 mm
- Manufacturer-Listed Height: 165 mm
- Exact Calibrated Volume: Not specified in the reviewed product record
- Brimful Capacity: Not separately specified
- Equipment Clearance: Evaluate the bottle together with the installed stopper and any accessory used in the laboratory.
Dimensional distinction: DWK lists the 227497-00 stoppered bottle as 69 × 165 mm but lists the 227498 bottle without stopper as 69 × 143 mm. Do not substitute bottle-only height data into the product setup for 227497-00.
USP Type I borosilicate glass: material properties and qualification limits
DWK identifies the WHEATON 227497-00 BOD bottle as manufactured from USP Type I borosilicate glass. This classification indicates a chemically durable glass type commonly used in laboratory and pharmaceutical-related container applications.
DWK's technical glass guide distinguishes multiple borosilicate compositions, including 3.3-, 4.9-, and 5.4-expansion glass categories. USP Type I classification alone does not establish which exact expansion formulation has been used for every product.
- Confirmed Material: Borosilicate glass
- Confirmed Classification: USP Type I
- Glass Stopper: Ground-glass robotic style, included
- Exact Expansion Grade: Not identified in the reviewed SKU specification
- ASTM E438 Subclassification: Not specifically stated in the current product listing
- Lot-Specific Certification: Not established by the product listing alone
- Volumetric Class A Calibration: Not identified; this product is an incubation bottle rather than a certified volumetric measuring instrument.
Specification control: Do not automatically describe 227497-00 as borosilicate glass 3.3 or ASTM E438 Type I, Class A unless an applicable product-specific manufacturer record confirms that designation.
Review DWK's Glass Types & Properties
Cleaning and contamination control for BOD measurements
BOD testing is sensitive to substances introduced during sample handling and glassware preparation. Residual detergents, oxidizing chemicals, organic contamination, and other cleaning residues may influence microbial activity or dissolved oxygen measurements.
DWK recommends washing laboratory glassware before first use and inspecting for visible damage. The actual cleaning method for BOD bottles should be the one specified by the laboratory's analytical procedure.
- Before First Use: Clean and inspect the bottle and glass stopper.
- After Testing: Remove sample residues promptly using a compatible procedure.
- Cleaning Agents: Use agents permitted by the approved BOD method and facility SOP.
- Rinsing: Use the water quality and rinse procedure appropriate to the analytical method.
- Stopper Joint: Keep ground-glass contact surfaces clean and free from damage.
- Storage: Protect prepared bottles from contamination and physical damage.
- Cleaning Verification: Follow any required checks for residues or cleaning effectiveness.
Analytical contamination rule: Do not assume that ordinary washing is sufficient for a sensitive analytical method. Residual chemicals can alter the biological oxygen consumption being measured. Follow the BOD-specific cleaning and rinsing procedure.
Review DWK's General Glassware Cleaning Advice
Sterility, autoclaving, and other processing claims
The reviewed DWK product record does not identify 227497-00 as supplied sterile and does not provide a SKU-specific validated autoclave cycle or sterilization procedure. The glass material's general thermal durability is not a substitute for finished-product processing instructions.
Likewise, the product listing does not establish a sterility assurance level, endotoxin limit, certified cleanroom cleanliness classification, or sterile packaging configuration. These characteristics should not be inferred from the USP Type I glass classification.
- Supplied Sterility: Not established
- Validated Autoclave Cycle: Not identified in the reviewed SKU record
- Endotoxin Specification: Not identified
- ISO Cleanroom Cleanliness: No product-specific classification identified
- Pressure or Vacuum Rating: Not established
- Processing Qualification: Must follow the laboratory procedure and any applicable manufacturer guidance.
Processing boundary: Do not publish a blanket autoclavability, sterile, or pressure-resistant claim for 227497-00 without explicit product-specific manufacturer confirmation. Avoid heating a closed glass-stoppered assembly under conditions that could create hazardous pressure differentials.
Unnumbered versus manufacturer-numbered and barcoded BOD bottles
The 227497-00 configuration has no manufacturer-assigned number sequence or barcode. It retains the large screened-on writing area for user-defined sample identification.
Other WHEATON 300 mL robotic-stopper bottles are supplied with preassigned number sequences and barcoding to support laboratories that use structured bottle identification or compatible automated sample-tracking processes.
| DWK SKU |
Stopper |
Number Sequence |
Barcode |
Quantity |
| 227497-00 |
Robotic Glass |
Unnumbered |
No |
24 |
| 227497-01 |
Robotic Glass |
01–24 |
Yes |
24 |
| 227497-02 |
Robotic Glass |
25–48 |
Yes |
24 |
| 227497-03 |
Robotic Glass |
49–72 |
Yes |
24 |
| 227497-00G |
Pennyhead Glass |
Unnumbered |
No |
24 |
Manufacturer-numbered configurations may be useful for laboratories using predefined bottle identification sequences. The unnumbered 227497-00 should be selected when user-assigned identification is preferred.
Replacement robotic glass stopper: WHEATON 227670
DWK identifies WHEATON 227670 as the replacement glass robotic stopper for catalog 227497-00. The manufacturer states that its BOD replacement stopper family can be used with WHEATON 60 mL and 300 mL BOD bottles.
The separately sold 227670 replacement part is described as a robotic-style glass stopper and is supplied in a manufacturer case quantity of 12 stoppers.
- Replacement Robotic Stopper: 227670
- Material: USP Type I borosilicate glass
- Stopper Style: Robotic
- Manufacturer Replacement Quantity: 12
- Alternate Pennyhead Replacement: 227672, a separate stopper style
- Compatibility: Manufacturer-described fit for the applicable WHEATON 60 mL and 300 mL BOD bottle family.
Replacement control: Glass stoppers are replaceable, but the fit and condition of the stopper joint must still be checked. Do not force a damaged or incompatible glass stopper into the bottle neck.
Packaging, receiving, and inspection checklist
The manufacturer specifies 24 BOD bottles per case for 227497-00. Because these are glass bottles supplied with precision-fitting glass stoppers, receiving procedures should verify both bottle identity and physical condition.
- Verify SKU: Match 227497-00 to the purchase order.
- Verify Capacity: Confirm the 300 mL bottle designation.
- Verify Quantity: Confirm the manufacturer case quantity of 24.
- Check Stopper Style: Confirm that the supplied glass stoppers are the robotic configuration.
- Check Numbering: Verify the bottles are unnumbered and not barcoded.
- Inspect Bottle Bodies: Look for cracks, chips, scratches, or other damage.
- Inspect Glass Joints: Check the bottle mouths and stopper surfaces for damage or contamination.
- Check Writing Patches: Confirm the marking areas are present and usable.
- Verify Equipment Fit: Confirm compatibility with racks, incubators, and dissolved oxygen measurement equipment.
- Record Disposition: Document receipt and acceptance according to the laboratory's quality system.
Receiving checkpoint: The manufacturer's 165 mm height is associated with the stoppered BOD bottle SKU. The 143 mm height listed for the separate bottle-only configuration should not replace this specification.
Technical FAQs
Does WHEATON 227497-00 include the glass stopper?
Yes. It is supplied with a robotic-style glass stopper. The manufacturer lists 24 bottles per case.
Are the bottles numbered or barcoded?
No. Catalog 227497-00 is unnumbered and not barcoded. The bottle has a permanently screened-on writing patch for identification.
What is the correct replacement stopper?
DWK specifies WHEATON 227670, a separately sold robotic glass stopper supplied in a case quantity of 12.
What is the difference between 227497-00 and 227497-00G?
227497-00 includes a robotic glass stopper. 227497-00G includes a pennyhead glass stopper. Both are 300 mL, unnumbered BOD bottles.
What is the difference between 227497-00 and 227498?
227497-00 includes the robotic glass stopper and has a published height of 165 mm. 227498 is the separate bottle-only configuration without a stopper and has a published height of 143 mm.
Why is the bottle mouth flared?
The flared mouth provides an area where a water seal can be maintained around the inserted stopper, helping prevent outside air from entering during incubation.
Why is the stopper tapered?
The tapered underside is designed to reduce air entrapment when the stopper is inserted into the filled bottle.
What does BOD5 mean?
BOD5 is the conventional five-day biochemical oxygen demand test. It measures oxygen depletion associated with microbial activity under defined laboratory incubation conditions.
Is the bottle compatible with all dissolved oxygen probes?
No universal compatibility guarantee is provided. DWK describes compatibility with the probes of leading BOD and dissolved oxygen meters, but the exact instrument and probe should be checked.
Is the 300 mL capacity individually calibrated?
The reviewed manufacturer record lists 300 mL nominal capacity but does not provide an individual calibrated volume tolerance. Follow the analytical method when actual bottle volume must be determined.
Does USP Type I mean the glass is specifically borosilicate 3.3?
Not necessarily. The manufacturer confirms USP Type I borosilicate glass for this SKU but does not specify its expansion grade in the reviewed product listing.
Does the bottle arrive sterile?
The reviewed DWK specification does not establish that this product is supplied sterile.
Can these BOD bottles be autoclaved?
A product-specific autoclavability claim and validated processing cycle were not identified in the reviewed manufacturer listing. Obtain confirmation for the exact bottle-and-stopper assembly before relying on an autoclave procedure.
Is a water-seal cover included?
The reviewed SKU record identifies a bottle with robotic glass stopper. It does not establish an additional water-seal overcap as included. Any cover required by the method should be arranged separately.
How many bottles are supplied per case?
The standard manufacturer case contains 24 BOD bottles with robotic glass stoppers.
Why purchase WHEATON BOD bottles from SOSCleanroom
- Specialty laboratory sourcing: SOSCleanroom supports environmental, analytical, and critical-environment laboratory procurement.
- Exact SKU identification: Our team can help distinguish robotic, pennyhead, unnumbered, barcoded, and bottle-only configurations.
- Replacement-part sourcing: Request assistance identifying the correct robotic glass stopper and other applicable laboratory supplies.
- Manufacturer documentation: Contact SOSCleanroom for available product information and specification requests.
- Procurement consolidation: Source laboratory glassware and related consumables through SOSCleanroom.
- Responsive customer service: Request quotations, availability, or product identification assistance from our team.
Manufacturer documentation and authoritative technical references
-
DWK Glass Types & Properties:
Review Manufacturer Glass Material Guidance
— general material classifications, chemical resistance, and glass properties.
-
DWK Care & Maintenance of Laboratory Glassware:
Review Manufacturer Glassware Care Guidance
— inspection, safe handling, glass-joint care, and general processing precautions.
-
DWK General Glassware Cleaning Advice:
Review Manufacturer Cleaning Guidance
— general cleaning and rinsing recommendations for laboratory glassware.
-
Analytical Method: Standard Methods for the Examination of Water and Wastewater, Method 5210B, Five-Day BOD Test. DWK references the 21st Edition (2005); use the applicable current method and laboratory procedure.
The manufacturer's individual 227497-00 specifications were used to verify the 300 mL capacity, glass robotic stopper, 69 mm diameter, 165 mm height, unnumbered status, absence of barcoding, replacement stopper 227670, and 24-bottle case quantity.
For product-specific material records, additional processing information, or assistance sourcing WHEATON BOD bottle accessories, contact SOSCleanroom.
If you have any questions please email us at Sales@SOSsupply.com or give us a call at (214) 340-8574.
For additional product guidance, use the SOSCleanroom AI ChatBot or contact our team for manufacturer documentation assistance.
Technical insight: Accurate BOD measurements depend on controlled sample preparation, correct dissolved oxygen measurement, minimized air entrapment, a maintained water seal, and proper incubation. The WHEATON BOD bottle supports these requirements through its specialized glass geometry and included stopper.
Last updated: October 28, 2026
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